Premature flowering in bananas , where the plant flowers earlier than expected, can disrupt fruit production and yield. Below are the key reasons for this phenomenon, based on available information: 1. Genetic Factors - Certain banana cultivars or…
NanoDrop spectrophotometer for DNA quality Analysis The NanoDrop spectrophotometer is a widely used tool for quantifying and assessing the quality of DNA, RNA, and proteins in small sample volumes (1–2 µL). It measures the absorbance of ultraviolet…
MAMP -Microbe associated molecular pattern / PAMP- Pathogen Associated molecular pattern By using pattern recognition receptors (PRRs) on the cell surface to identify and activate microbe-associated molecular patterns (MAMPs), plants are able to ident…
the basic bioinformatic tools for viral genome analysis Several fundamental bioinformatic instruments are frequently employed for viral genome study, including: 1. Viral genome sequences can be aligned with other sequences or with reference sequences …
PROCEDURE FOR RT-PCR The following is a general procedure for performing a reverse transcriptase polymerase chain reaction (RT-PCR): 1. RNA extraction: RNA is extracted from the biological sample (e.g. blood, tissue, or cell culture) using an appro…
GENOTYPIC TOOLS SUCH AS GENOME/ SPECIFIC GENE SEQUENCE HOMOLOGY COMPARISON BY BLAST (NCBI AND EMBL) BLAST Basic Local Alignment Search Tool (BLAST) is the tool most frequently used for calculating sequence similarity. BLAST comes in va…